Fig. 3
From: Modular plasmid design for autonomous multi-protein expression in Escherichia coli
Relative expression from RhaS/RhaBAD, LacI/Trc, AraC/AraBAD and XylS/Pm cassettes in comparison to constitutive expression of mCherry at 6, 12, 18 and 24 h of the cultivation. Inducers concentrations were: 10 mM rhamnose, 0.5 mM IPTG, 10 mM arabinose, and 0.0025 mM m-toluic acid. The results of unpaired t-test between constitutive and induced mCherry expression are showed in the Table S3. Error bars represent the standard deviations of 6 individual replicates. Fluorescence values were normalised against the OD600 and compared to the normalised fluorescence values of constitutively expressed mCherry. Plain E. coli BL21 (DE3) strain was used as a control