Fig. 5

Influence of the carbon source on the induction. The differences in fluorescence intensity of mCherry (bar-plot) and OD₆₀₀ (cross-plot) at 18 h of cultivation between cultures supplemented with 0.8% glucose or 0.8% glycerol were analysed by unpaired t-test (Table S6 and Table S7). Strains were cultured at 37 °C for 24 h in 2xM9 minimal medium. Inducers concentrations were: 10 mM rhamnose, 1 mM IPTG, 10 mM arabinose, and 0.0025 mM m-toluic acid. Error bars represent the standard deviations of 6 individual replicates. Fluorescence values were normalised against the OD₆₀₀. Plain E. coli BL21 (DE3) strain was used as a control