Fig. 9
From: Modular plasmid design for autonomous multi-protein expression in Escherichia coli
Production of flavonoid rhamnosylation cascade A SDS-PAGE gel showing co-expressed GmSuSy, VvRHM_NRS/ER and AtUGT89C1 purified on His SpinTrap columns from cell lysate of differently induced. E. coli harbouring pTriple_cascade (Lane 3–6) or pRhaS_cascade (Lane 2) plasmid. Lane 1 – PageRuler Plus Prestained Protein Ladder; Lane 3 – rhamnose induction; Lane 4 – IPTG induction; Lane 5 - m-toluic acid induction; Lane 6 – induction with all three inducers. Proteins mass – GmSuSy – 92 kDa, VvRHM_NRS/ER – 76 kDa, AtUGT89C1 –48 kDa. B Conversions of 0.05 mM naringenin to naringenin 7-O-rhamnoside conducted using corresponding from SDS-PAGE gel cell lysates