Nanozyme-based aptasensors for the detection of tumor biomarkers

Table 4 Single-mode aptamer biosensors based on nanozymes for other protein tumor biomarkers detection

Cancer type	Biomarker	Aptamer	Nanozyme	Substrate	Detection Method	Electrode	Linear Range	LOD	Sensitivity	Recovery (RSD)	Assay Time	Ref.
−	HER2	HER2	Mn₃O₄ Pd@Pt	HQ/H₂O₂	DPV	GE	0.1 − 100 ng/mL	0.08 ng/mL	−	−	−	[73]
Ovarian Cancer	CA125	Aptamer 2.26 Aptamer 2.43	Au	DAB//H₂O₂	ALFA	−	7.5 − 200 U/mL	5.21 U/mL	−	86.5 − 114.8%	−	[75]
Ovarian Cancer	CA125	CA125	CeO₂	TMB/H₂O₂	Colorimetry	−	1.0 − 10.0 U/mL	0.43 mU/mL	−	95.4–104.0% (< 6.7%)	−	[76]
HCC	GP73	GP73	RGO-CMCS-Hemin/Pt@Pd	TMB/H₂O₂	Colorimetry	−	10.0–110.0 ng/mL	4.7 ng/mL	−	98.2–107.0% (1.90–5.44%)	120 min	[78]
	GP73	GP73	H-rGO-Mn₃O₄	TMB/H₂O₂	SWV	SPE	0.01–100.0 ng/mL	0.0071 ng/mL	2.441 µA/µM/cm²	98.66–121.11%	−	[79]
	GPC3	GPC3	H-R-Pt@Pd	TMB/H₂O₂	Colorimetry	−	10–300 ng/mL	5.06 ng/mL	−	105.94–116.33% (0.93– 3.08%)	−	[80]
	GPC3	GPC3	H-rGO-Pd	AgNO₃/ H₂O₂	DPV	SPE	0.01–10.0 µg/mL 10.0–100.0 µg/mL	3.30 ng/mL	1.535 µAµM^− 1 cm^− 2	103.78–106.52% (1.89–8.81%)	30 min	[81]
−	VEGF₁₆₅	VEGF₁₆₅	(MnCo)Fe₂O₄	4-CN/H₂O₂	PEC	FTO	1 × 10^− 2−1 × 10⁴ pg/mL	0.1 fg/mL	−	100.9–101.7% (2.12–2.15%)	60 min	[83]

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