Table 5 Dual-mode nanozyme-based aptamer biosensors for tumor biomarker detection
From: Nanozyme-based aptasensors for the detection of tumor biomarkers
Biomarker | Aptamer | Nanozyme | Substrate | Detection Method | Electrode | Linear Range | LOD | Recovery (RSD) | Assay Time | Ref. |
|---|---|---|---|---|---|---|---|---|---|---|
MCF-7 CTCs | SYL3C | MOF@Pt@MOF | TMB/H2O2 | SWV Colorimetry | Au/rGO/ITO − | 5 − 5 × 105 cells/mL | 5 cells/mL | 100.0 − 109.0% (0.6 − 2.3%) | − | [91] |
MCF-7 exosomes | CD63 EpCAM | TMB-GQDzymes | TMB/H2O2 | Colorimetry Photothermal analysis | − | 0 − 4 × 104 particles/µL 2 × 103−4 × 104 particles/µL | 1027 particles/µL 2170 particles/µL | − | − | [66] |
CD63 | MoS2- Au@Pt | TMB/H2O2 | Chronoamperometry Colorimetry | Au − | 10 − 109 particles/mL 104−109 particles/mL | 9.3 particles/mL 4.2 × 103 particles/mL | > 93% (< 8%) | − | [92] | |
PSA | PSA | PtNP@Co3O4 | TMB/H2O2 | DPV Colorimetry | SPE − | 0.01 − 10 ng/mL 0.01 − 15 ng/mL | 0.0079 ng/mL 0.01 ng/mL | 97.03 − 105.75% | − | [93] |