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Table 3 Average values of key production parameters obtained from the exponential methanol feed phase of fed-batch cultivations using a pre-programmed µ of 0.03 h-1. Volumetric productivity (QP), biomass yield on methanol (YX/S), 3-HP yield on methanol (YP/S), 3-HP yield on biomass (YP/X), CO2 yield on methanol (YCO2/S), specific substrate consumption rate (qS), specific 3-HP production rate (qP), specific carbon dioxide evolution rate (qCO2), and experimentally measured mean specific growth rate (µ). Cultivations were performed in duplicate and biomass concentration analyses were performed in triplicate. ± indicates SD of the biological replicates

From: Metabolic engineering of Komagataella phaffii for enhanced 3-hydroxypropionic acid (3-HP) production from methanol

 

PpCβ21-P

PpCβ21-PEJY

QP (g3 − HP l− 1 h− 1)

0.46 ± 0.01

0.56 ± 0.03

YX/S (gDCW gMetOH−1)

0.21 ± 0.01

0.25 ± 0.01

YP/S (g3 − HP gMetOH−1)

0.15 ± 0.01

0.19 ± 0.01

YP/X (g3 − HP gDCW−1)

0.69 ± 0.03

0.74 ± 0.02

YCO2/S (gCO2 gMetOH−1)

0.81 ± 0.03

0.68 ± 0.02

qS (gMetOH gDCW−1 h− 1)

0.124 ± 0.003

0.109 ± 0.001

qP (mmol3 − HP gDCW−1 h− 1)

0.201 ± 0.008

0.227 ± 0.006

qCO2 (mmolCO2 gDCW−1 h− 1)

2.30 ± 0.08

1.69 ± 0.04

µ (h− 1)

0.026 ± 0.001

0.028 ± 0.001

Reference

[39]

This study