Table 2 Isotopic labeling experiments from ¹³CO₂ demonstrate the preferential TCA cycle metabolites synthesis and metabolites concentration in transgenic E. Coli K12

Strain VC³

42.89 ± 12.27

0.048 ± 0.024

637.39 ± 121.82

0.0020 ± 0.0018

Strain K⁴

36.89 ± 14.14

0.079 ± 0.001

499.13 ± 179.39

0.0014 ± 0.0017

P-value⁶

0.584

0.074

0.306

0.696

Strain KA⁵

30.15 ± 4.36

0.032 ± 0.023

404.89 ± 123.97

0.0019 ± 0.0018

P-value⁶

0.165

0.417

0.081

0.981

KA vs. K⁷

0.470

0.016

0.474

0.715

α-KG

α-KG + 1

Suc

Suc + 1

Strain VC

0.35 ± 0.07

0.010 ± 0.009

2.67 ± 0.75

0.0001 ± 0.0002

Strain K

0.27 ± 0.12

0.135 ± 0.023

2.83 ± 0.41

0.1387 ± 0.0487

P-value

0.346

< 0.001

0.719

0.002

Strain KA

0.24 ± 0.06

0.010 ± 0.009

1.70 ± 0.11

0.0020 ± 0.0034

P-value

0.097

0.604

0.075

0.303

KA vs. K

0.715

0.005

0.006

0.008

Fum

Fum + 1

Mal

Mal + 1

Strain VC

0.48 ± 0.21

0.008 ± 0.0001

1.16 ± 0.50

0.002 ± 0.002

Strain K

0.78 ± 0.11

0.022 ± 0.002

2.43 ± 0.39

0.023 ± 0.001

P-value

0.089

< 0.001

0.013

< 0.001

Strain KA

0.75 ± 0.08

0.019 ± 0.008

2.82 ± 0.46

0.006 ± 0.008

P-value

0.105

0.056

0.013

0.431

KA vs. K

0.703

0.499

0.281

0.007

Cit

Cit + 1

Cit + 2

Strain VC

1.57 ± 0.77

0.0003 ± 0.0005

0.003 ± 0.002

Strain K

6.11 ± 2.33

0.1338 ± 0.0386

0.012 ± 0.006

P-value

0.025

0.004

0.052

Strain KA

5.49 ± 0.70

0.0006 ± 0.0011

0.014 ± 0.008

P-value

0.001

0.659

0.086

KA vs. K

0.631

0.004

0.790