Carbon fluxes rewiring in engineered E. coli via reverse tricarboxylic acid cycle pathway under chemolithotrophic condition

Table 3 Isotopic labeling experiments from ¹³CO₂ demonstrate the preferential proteinic amino acid synthesis in transgenic E. Coli K12

	Asp + 1¹	Glu + 1	Lys + 1	Thr + 1
Strain VC²	0.017 ± 0.001	0.000 ± 0.001	0.011 ± 0.001	0.022 ± 0.002
Strain K³	0.037 ± 0.002	0.006 ± 0.001	0.019 ± 0.003	0.030 ± 0.000
P-value⁵	< 0.001	0.001	0.006	0.001
Strain KA⁴	0.052 ± 0.004	0.012 ± 0.002	0.019 ± 0.002	0.037 ± 0.003
P-value⁵	< 0.001	0.001	0.002	0.003
KA vs. K⁶	0.003	0.013	0.943	0.034
	Ile + 1	Met + 1	Gly + 1	Ser + 1
Strain VC	0.0005 ± 0.0005	0.004 ± 0.001	0.020 ± 0.001	0.024 ± 0.004
Strain K	0.0038 ± 0.0004	0.011 ± 0.000	0.028 ± 0.001	0.027 ± 0.002
P-value	0.001	0.001	0.001	0.316
Strain KA	0.0044 ± 0.0.0019	0.015 ± 0.001	0.025 ± 0.002	0.030 ± 0.002
P-value	0.026	< 0.001	0.0440	0.048
KA vs. K⁶	0.632	0.001	0.078	0.064
	Cys + 1	Ala + 1	Tyr + 1	Phe + 1
Strain VC	0.009 ± 0.001	0.0061 ± 0.0008	0.010 ± 0.000	0.0018 ± 0.0004
Strain K	0.016 ± 0.001	0.0056 ± 0.0004	0.027 ± 0.002	0.0044 ± 0.0002
P-value	0.001	0.418	< 0.001	0.001
Strain KA	0.049 ± 0.012	0.0080 ± 0.0015	0.015 ± 0.003	0.0082 ± 0.0026
P-value	0.004	0.134	0.001	0.014
KA vs. K⁶	0.008	0.063	0.003	0.067

Data are expressed as mean ± SD (n = 3/group) and compared by Student’s t-test
¹ The enrichments in Asp (aspartate), Glu (glutamate), Lys (lysine), Thr (threonine), Ile (isoleucine), Met (methionine), Gly (glycine), Ser (serine), Cys (cysteine), Ala (alanine), Tyr (tyrosine), Phe (phenylalanine) derived from ¹³CO₂ are shown
² Strain VC: E. coli K12 vector control
³ Strain K: E. coli K12 korAB (+) strain
⁴ Strain KA: E. coli K12 korAB (+) & aclBA (+) strain
⁵ Statistically significantly compared to the strain VC at p < 0.05; borderline statistical significance compared to strain VC at 0.05 < p ≦ 0.1
⁶ Statistically significantly compared to the strain K at p < 0.05; borderline statistical significance compared to strain K at 0.05 < p ≦ 0.1

ISSN: 1754-1611