Carbon fluxes rewiring in engineered E. coli via reverse tricarboxylic acid cycle pathway under chemolithotrophic condition

Table 4 Effect of hetero-expressed enzymes promote CO₂ incorporation into nucleotides in E. Coli K12

	dC + 1¹	dC + 2	dT + 1	dT + 2
Strain VC²	0.035 ± 0.003	0.041 ± 0.019	0.042 ± 0.002	0.019 ± 0.001
Strain K³	0.032 ± 0.003	0.035 ± 0.009	0.036 ± 0.001	0.016 ± 0.003
P-value⁵	0.231	0.647	0.027	0.142
Strain KA⁴	0.044 ± 0.004	0.045 ± 0.007	0.054 ± 0.005	0.018 ± 0.002
P-value⁵	0.024	0.783	0.016	0.386
KA vs. K⁶	0.009	0.226	0.004	0.430
	dG + 1	dG + 2	dA + 1	dA + 2
Strain VC	0.047 ± 0.003	0.000 ± 0.000	0.027 ± 0.001	0.004 ± 0.002
Strain K	0.080 ± 0.002	0.007 ± 0.001	0.049 ± 0.003	0.007 ± 0.001
P-value	< 0.001	< 0.001	< 0.001	0.131
Strain KA	0.101 ± 0.005	0.010 ± 0.003	0.063 ± 0.003	0.011 ± 0.002
P-value	< 0.001	0.005	< 0.001	0.009
KA vs. K⁶	0.002	0.166	0.006	0.021

Data are expressed as mean ± SD (n = 3/group) and compared by Student’s t-test
¹ The enrichments in dC (deoxycytidine), dT (deoxythymidine), dG (deoxyguanosine), dA (deoxyadenosine) derived from ¹³CO₂ are shown
² Strain VC: E. coli K12 vector control
³ Strain K: E. coli K12 korAB (+) strain
⁴ Strain KA: E. coli K12 korAB (+) & aclBA (+) strain
⁵ Statistically significantly compa-red to the strain VC at p < 0.05; borderline statistical significance compared to strain VC at 0.05 < p ≦ 0.1
⁶ Statistically significantly compared to the strain K at p < 0.05; borderline statistical significance compared to strain K at 0.05 < p ≦ 0.1

ISSN: 1754-1611