Table 2 The applied PCR primer sequences
|  | Forward primer sequence (5’-3’) | Reverse primer sequence (5’-3’) |
|---|---|---|
Osteocalcin (OC) | AAGAGACCCAGGCGCTACCT | AACTCGTCACAGTCCGGATTG |
Bone sialoprotein (BSP) | AACGAAAGCGAAGCAGAA | TCTGCCTCTGTGCTGTTGGT |
Osteopontin (OPN) | TGGAAAGCGAGGAGTTGAATG | CATCCAGCTGACTCGTTTCATAA |
Osteoprotegerin (OPG) | GTCAAGCAGGAGTGCAATCG | TAGCGCCCTTCCTTGCATT |
Osteonectin (ON) | GACTACATCGGGCCTTGCAA | GAGTGTGTGCCCACTGAGGAGTCCAAC |
Vascular endothelial growth factor (VEGF) | GAGTGTGTGCCCACTGAGGAGTCCAAC | CTCCTGCCCGGCTCACCGCCTCGGCTT |
Collagen type I (COL1A1) | GTCTAGACATGTTCAGCTTTGTGGA | CTTGGTCTCGTCACAGATCACGTCAT |
Bone morphogenetic protein 2 (BMP-2) | TGCTAGTAACTTTTGGCCATGATG | GCGTTTCCGCTGTTTGTGTT |
Noggin (NOG) | GCGCTGCGGCTGGAT | AGCACTTGCACTCGGAAATGA |
TBP (reference gene) | GGAGCTGTGATGTGAAGTTTCCTA | CCAGGAAATAACTCTGGCTCATAAC |