Fig. 3
From: Gene syntaxes modulate gene expression and circuit behavior on plasmids
Gene orientation relative to the plasmid Ori affects gene expression profiles. (A, D) In each plasmid pair, two reporter genes, placed at the same location on the corresponding plasmid, face the same direction either both codirectional to or both opposite to the plasmid Ori (ColE1). The mean levels and ratios of gene expression were compared. (B, C) Contribution of upstream DNA sequences to the transcription of GFP (B) or RFP (C) on plasmids DA464 and DA463, respectively. (E, F) Contribution of upstream DNA sequences to the transcription of GFP (E) and RFP (F) on plasmids DA461 and DA465. Note that transcription rates were evaluated using the Promoter Calculator, a reliable computational tool for identifying promoters and predicting their activity. Values are presented as mean ± SD (n = 8). *** p < 0.0001; ** p < 0.01; * p < 0.05; ns, not significant