Fig. 4

CRISPRi-assisted metabolic engineering of S. elongatusstrains for photosynthetic HA production (a) Cell growth of various strains used for CRISPRi-assisted metabolic engineering (Table 1). Cell growth was measured at OD₇₃₀ after 15 d cultivation. Data represent mean values of at least triplicated cultivations, and error bars represent standard deviations. (b) Photosynthetic HA production from engineered strains. Open bar; supernatant, filled bar; cell. Data represent mean values of at least triplicated cultivations, and error bars represent standard deviations. A Student’s t-test was performed for statistical analysis (*p-value < 0.05; **p-value < 0.005, ***p-value < 0.0005). Abbreviation: G3P, glyceraldehyde 3-phosphate; F6P, fructose 6-phosphate; GlcN-6P, glucosamine 6-phosphate; UDP-GlcNAc-1P, UDP-N-acetylglucosamine 1-phosphate; G6P, glucose 6-phosphate; G1P, glucose 1-phosphate; 6PG; UDP-GlcA, UDP-glucuronic acid