Table 1 Bacterial strains and plasmids used in this study
From: CRISPRi-assisted metabolic engineering of cyanobacteria for photosynthetic hyaluronic acid from CO2
Strain | Relevant characteristics | References |
|---|---|---|
E. coli DH5α | F−(80d lacZ M15) (lacZYA-argF) U169 hsdR17(r– m+) recA1 endA1 relA1 deoR96 | [39] |
S. elongatus PCC 7942 | Synechoccous elongatus PCC 7942, Wild type | PCC |
SeHA100 | S. elongatus PCC 7942 NSI::Bb1s-HasA, the hasA(co) originated from Streptomyces zooepidemicus | This study |
SeHA200 | S. elongatus PCC 7942 NSI::Bb1s- HasA-HasB-HasC, the hasA(co), the hasB(co) originated from S. pyogenes, the hasC(co) originated from S. pyogenes | This study |
SeHA210 | SeHA200 NSII::Bb1k-glmU-glmM, the glmU(co), glmM(co) originated from E. coli | This study |
SeHA220 | SeHA200 NSII::Bb1k-glmU-glmM-glmS, the glmS(co) originated from E. coli | This study |
Se3R-none | S. elongatus NSIII::Bb1c-dCas12a(co) | [24] |
SeHA211 | SeHA210 NSIII::Bb1c-dCas12a(co) | This study |
SeHA212 | SeHA210 NSIII::Bb1c-dCas12a(co)-nagB, native nagB gene repression by dCas12a-crRNA(nagB) | This study |
SeHA213 | SeHA210 NSIII::Bb1c-dCas12a(co)-glgC, native glgC gene repression by dCas12a-crRNA(glgC) | This study |
SeHA214 | SeHA210 NSIII::Bb1c-dCas12a(co)-zwf, native zwf gene repression by dCas12a-crRNA(zwf) | This study |
SeHA215 | SeHA210 NSIII::Bb1c-dCas12a(co)-pfk, native pfk gene repression by dCas12a-crRNA(pfk) | This study |
SeHA221 | SeHA220 NSIII::Bb1c-dCas12a(co) | This study |
SeHA222 | SeHA220 NSIII::Bb1c-dCas12a(co)-nagB, native nagB gene repression by dCas12a-crRNA(nagB) | This study |
SeHA223 | SeHA220 NSIII::Bb1c-dCas12a(co)-glgC, native glgC gene repression by dCas12a-crRNA(glgC) | This study |
SeHA224 | SeHA220 NSIII::Bb1c-dCas12a(co)-zwf, native zwf gene repression by dCas12a-crRNA(zwf) | This study |
SeHA225 | SeHA220 NSIII::Bb1c-dCas12a(co)-pfk, native pfk gene repression by dCas12a-crRNA(pfk) | This study |
SeHA226 | SeHA220 NSIII::Bb1c-dCas12a(co)-zwf-pfk, native zwf and pfk gene repressions by dCas12a-crRNA(zwf)-crRNA(pfk) | This study |