Fig. 1

Characterization of the ΔminCD strain. A The MinCD complex ensures symmetrical cell division by localizing properly at the cell poles. In its absence, irregular division occurs, leading to minicell formation. B Phase-contrast microscopy images of the ΔminCD strain. Cells grown overnight in LB were visualized using a phase-contrast microscope. Scale bar, 10 µm. C Representative flow cytometry dot plots showing side scatter (SSC) versus forward scatter (FSC) for WT and ΔminCD strains. Cells were cultured overnight in LB, and more than 50,000 events were analyzed. Purified minicells were also included in the analysis