Fig. 5

Engineering of the spatial distribution of reporter protein using polar localization signals. A Schematic representation of constructs designed to enable the potential repositioning of the reporter protein. Polarly localized RNA elements, ArcZ sRNA and segmented rpoS mRNA, were transcriptionally fused to the reporter gene. Translational fusions with proteins exhibiting polar localization, PtsI and Tsr, were also included. B Reporter strains carrying different fusion constructs were cultured in the presence of 10 µM IPTG, and fluorescence intensities were measured in isolated parent cells and minicells. C The fluorescence signals from (B) were analyzed to evaluate the relative enrichment of the reporter protein in minicells compared to parent cells. Fold enrichment in minicells is calculated as described in Fig. 4C. Error bars represent the mean ± standard deviation (n = 3), ns; not significant; *P < 0.1, compared to the non-fusion condition