Fig. 6

PopZ protein-mediated positioning of the reporter protein in minicells. A Schematic representation of the IPTG inducible mRFP-PopZ construct. B The reporter strain, carrying the PopZ fusion, was cultured with varying concentrations of IPTG (0, 10, 20, and 50 µM). The fluorescence intensities were measured in both isolated parent cells and minicells. C The RFP signals from each fraction were used to estimate the fold enrichment of the reporter protein in minicells compared to parent cells. Error bars represent mean ± SD (n = 3), **P < 0.05, compared to the no IPTG treated condition. D The localization of the PopZ-RFP fusion protein was visualized in both parent cells and minicells through microscopy. Images were captured in phase contrast and fluorescence channels and merged using ImageJ. Scale bars, 10 µm