Fig. 4

0.9 UCOE induces gene leakage and unintended differentiation of iPS cells. A. Analysis of FOXN1 gene leakage in pre-iTEC1 and PGP1 without doxycycline treatment (n = 3). B. RT-qPCR comparison of undifferentiated marker genes OCT4, NANOG, and DNMT3 in pre-iTEC1 and PGP1 cultured without doxycycline for 30 days (n = 3). C. RT-qPCR comparison of TEC-specific markers DLL4 and ENPEP (Ly51) in pre-iTEC1 during 90 days culture without doxycycline (n = 3). D. Analysis of FOXN1 leakage in bulk cell populations of the 0.9 UCOE and w/o UCOE group by RT-qPCR (n = 3). E. FOXN1 leakage and ENPEP (Ly51) expression analysis in single-cell-derived colonies of 0.9 UCOE group and w/o UCOE group by RT-qPCR (n = 10). P-values were calculated using a two-tailed unpaired t-test and one-way analysis of variance with Tukey’s honestly significant difference test. Data are presented as mean ± SEM. For detailed data, statistical analyses, and exact p-values, see the source data file