Fig. 1

CBE system in Vibrio sp. dhg (A) Overview of the CBE mechanism, comprising the coordinated actions of dCas9/sgRNA, CDA, and UGI in C-to-T base conversion. (B) Schematic diagrams of the plasmids for the expression of dCas9-CDAL (pCBE1) or dCas9-CDA-UGIL (pCBE2) and sgRNA (psgRNA series)