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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Development of a CRISPR-based cytosine base editor for restriction-modification system inactivation to enhance transformation efficiency in Vibrio Sp. dhg

Fig. 2

Single target base editing efficiency characterization. (A) Sequence outcome of single target base editing targeting rpoB and pyrF using the CBE1(without UGI) and CBE2(with UGI) system. Eight (or sixteen for the CBE1 system, rpoB target) random colonies were selected and analyzed to calculate editing efficiency as the ratio of colonies containing targeted mutagenesis. (B) Comparison of single target base editing efficiency between CBE1 (without UGI) and CBE2 (with UGI)

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Journal of Biological Engineering

ISSN: 1754-1611