Table 1 Relative initial on- and off-rates for hFcRn binding of trastuzumab and trastuzumab Fc variants at the initial phases of association (pH 6.0) and dissociation (pH 7.4)
| Â | Association at pH 6.0 | Dissociation at pH 7.4 | Average of relative on- and off-rate | Rank | ||
|---|---|---|---|---|---|---|
Relative on-rate | R2 | Relative off-rate | R2 | |||
Trastuzumab | 1.000±0.000 | 0.9982 | 1.000±0.000 | 0.9995 | 1.000±0.000 | 5 |
Trastuzumab-DHS | 1.809±0.054 | 0.9978 | 1.831±0.071 | 0.9994 | 1.820±0.011 | 2 |
Trastuzumab-PFc29 | 1.940±0.094 | 0.9977 | 1.598±0.123 | 0.9996 | 1.769±0.171 | 3 |
Trastuzumab-YML | 2.105±0.119 | 0.9979 | 1.737±0.125 | 0.9996 | 1.921±0.184 | 1 |
Trastuzumab-EML | 1.472±0.067 | 0.9978 | 1.497±0.084 | 0.9994 | 1.484±0.012 | 4 |