Table 1 Human-specific primer pairs designed for the evaluation of target molecule expression
From: Anti-inflammatory activity of magnetic fields emitted by graphene devices on cultured human cells
Gene | Forward Sequence (5’-3’) | Reverse Sequence (5’-3’) |
|---|---|---|
TNF-α | CCTTCCTGATCGTGGCAG | GCTTGAGGGTTTGCTACAAC |
IL-1β | TGAGGATGACTTGTTCTTGAAG | GTGGTGGTCGGAGAATG |
IL-6 | GAGCTGTGCAGATGATGAGTACAA | GGACTGCAGGAACTCCTTAAA |
IL-10 | TTTAAGCTGTTTCCATAGGGTGA | TTTATCTTGTCTCTGGGCTTGG |
iNOS | CATTGCTGTGCTCCATAGTTTC | CAGGACGTAAGTTCAGCATCTC |
COX-2 | CGATGCTGTGGAGCTGTAT | TTGAGGCAGTGTTGATGATTTG |
18s | CTTTGCCATCACTGCCATTAAG | TCCATCCTTTACATCCTTCTGTC |