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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management

Fig. 2

Analysis of sHRP and cHRP secretion and catalyzation ability. A Protein sequence schematics, SP of cHRP was removed to prevent secretion. B Molecular weight comparison by western blotting, Lane 1; 293 T cells transfected with cHRP (293 T-cHRP), Lane 2; 293 T cells transfected with sHRP (293 T-sHRP), Lane 3; untransfected 293 T cells as a negative control. Actin was used as the loading control. C-D ( , or ) for ABTS group and ( , or ) for the TMB group. All x-axes were converted into an exponential of 2, n = 3, with 3 independent repeats

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Journal of Biological Engineering

ISSN: 1754-1611