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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management

Fig. 3

Comparison of the sensitivity of sHRP and the CLuc system. A Protein sequence schematics of pTEAD1-sHRP, pTEAD1-CLuc, pAP- 1-sHRP, and pAP- 1-CLuc. B-M Signal sensitivity of sHRP and CLuc with different substrates, ( and ) sHRP-ABTS, ( and ) sHRP-TMB, ( and ) CLuc-vargulin, B-G plasmid dilution assays, B-D using pTEAD1 plasmid, E–G using pAP- 1 plasmid. H-M supernatant dilution assays, H-J with pTEAD1, K-M with pAP- 1. All the error bars show SD, n = 3, with 3 independent repeats

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