Skip to main content
Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management

Fig. 4

Stability analysis of sHRP-ABTS, TMB, and CLuc-vargulin. Signal stability analysis, A-C serum group, D-F SFM group. ( and ) sHRP-ABTS, ( and ) sHRP-TMB, ( and ) CLuc-vargulin. Enzyme stability analysis, G-J sHRP-ABTS group, K-N sHRP-TMB group, O-R CLuc-vargulin group. ( 0 h, 24 h, 48 h, 72 h, 96 h) enzymes with corresponding substrates, ( 0 h, 24 h, 48 h, 72 h, 96 h) supernatants from untransfected 293 T with substrates. All error bars show SD, n = 5 for the ABTS group, n = 6 for the TMB group, n = 3 for the CLuc group, with 3 independent repeats

Back to article page