Fig. 5

Simulation of a real-world reporter gene application. A Protein sequence schematics for the NF-κB response element (NF-κB-RE) plasmid containing sHRP. B-C Supernatants from 293 T cells transfected with NF-κB-sHRP, treated with serially diluted TNF-α (10 ng/mL to 0.156 ng/mL) and substrates (ABTS or TMB). The X-axis was converted in the exponential of 2. D-E Supernatants from 293 T-NF-κB-sHRP cells stimulated with 5 ng/mL TNF-α and assayed at 0, 3, 6, 9, and 12 h. ( ) Supernatants from 293 T-NF-κB-sHRP cells with TNF-α or ( ) without TNF-α. ( ) Supernatants from 293 T△NF-κB-sHRP cells with TNF-α or ( ) without TNF-α. All error bars showed SD, n = 3, with 3 independent repeats