Fig. 1
From: High-throughput strategies for monoclonal antibody screening: advances and challenges
The high-throughput antibody production scheme of the antibody library display technology. Antibody libraries from immunized animals and humans are displayed on a variety of vectors (e.g. phage, yeast, ribosomes, etc.) to construct diverse antibody libraries, and high-affinity binding vectors are isolated through multiple rounds of iterative screening. The gene sequences of the variable regions of the antibodies were determined by ELISA identification and sequencing analysis. The gene sequences of the variable regions are integrated into the designated expression vectors for antibody production, followed by downstream characterization and analysis