Fig. 2
From: High-throughput strategies for monoclonal antibody screening: advances and challenges
The high-throughput antibody production scheme of the single B cell antibody technology. In the context of isolating PBMCs or splenocytes from immunized animals, as well as PBMCs from convalescent patients, various techniques such as MACS can be employed to enhance the concentration of target B cells. Subsequently, antigen-specific single B cells be isolated using methods such as FACS and microfluidics. The antibody variable region genes can be cloned through RT-PCR to facilitate antibody expression and subsequent analysis