Fig. 3
From: High-throughput strategies for monoclonal antibody screening: advances and challenges
The high-throughput antibody production scheme of the single-cell sequencing. In the context of isolating PBMCs or splenocytes from immunized animals, as well as PBMCs from convalescent patients. Target B cells were enriched using MACS or FACS. Single-cell sequencing was conducted on the enriched cells to obtain 5'-end transcriptome data and immune repertoire data. Bioinformatics analysis was then employed to identify high-affinity antibody gene sequences from a vast amount of single-cell sequencing data. Specific vectors were subsequently incorporated for antibody expression and downstream characterization