Table 1 Characteristics of different antibody high-throughput preparation techniques
From: High-throughput strategies for monoclonal antibody screening: advances and challenges
Platform | Â | Throughput | Automation | Chain Pair | Affinity | Screening period | Potential improvements | Representative |
|---|---|---|---|---|---|---|---|---|
Antibody Library Display | Relatively low | Low | N | Low | 1–2 month | Affinity of antibodies; Library quality | Phage Display [15] | |
Single B-cell sorting platform | FACS | Medium (10, 000cell/s) | Low | Y | Relatively Lower | 16–18 weeks | Low screening specificity; Difficult to screen for ASCs | BD FACSAria™ [89] |
Microdroplet | High (millions cell/s) | Medium | Y | High | 4−6 weeks | False negatives; Limited droplet volume | Cyto-Mine [105] | |
Microwell | Medium (1–200,000/chip) | Low | Y | Medium | The technology is complex; contamination between cells | ISAAC [117] | ||
Micro-Chamber | Medium (1–20, 000/chip) | Hight | Y | High | Expensive; contamination between cells | Beacon® [125] | ||
Single-cell sequencing | 160,000cell/run | Hight | Y | High | 1–2 weeks | An efficient method for analyzing sequencing data | 10 × Genomic [144] | |