Fig. 2

Binding ability analysis of the scFv-masked bispecific antibody against (a) T cells and (b) cancer cells before and after collagenase digestion. Phosphate-buffered saline (PBS) was used as a negative control in each assay, and each antibody was incubated with CD3-positive lymphokine-activated killer cells with a T-cell phenotype (T-LAK) cells or epidermal growth factor receptor (EGFR)-positive TFK-1 cells. The cells were stained with a fluorescein isothiocyanate (FITC)-labeled anti-human antibody after incubation